We are seeking a highly motivated and detail-oriented Research Assistant to join the Steimle Lab cardiovascular research team. This role is ideal for candidates with strong experience in animal colony management, molecular genotyping, and laboratory compliance. The successful candidate will play a critical role in supporting ongoing research projects by maintaining our mouse colony, performing surgeries and experiments, and ensuring regulatory compliance.

🧬 Key Responsibilities

• 🐭 Animal Colony Management: Oversee the health, breeding, and documentation of a large mouse colony, including multiple genetic strains.

• 🧪 Genotyping & Sample Processing: Perform ear punching, tail clipping, DNA extraction, and PCR-based genotyping for various mouse lines.

• 🐁 Surgical Procedures & Experimental Work: Schedule and conduct small animal surgeries and experimental protocols with precision and care.

• 🧫 Histology & Imaging: Carry out histological staining and imaging to support tissue-based research.

• 📋 Regulatory Compliance: Manage and maintain documentation for IBC and IACUC protocols, ensuring full compliance with institutional and federal guidelines.

• 📊 Data Analysis & Reporting: Analyze experimental data and prepare detailed reports for the principal investigator.

• 🧾 Documentation & Inventory: Maintain accurate breeding records and manage the lab’s chemical inventory.

• 👩‍🔬 Training & Safety Oversight: Train new personnel in animal handling and equipment use; ensure adherence to lab safety protocols.

JOB RESPONSIBILITIES

• Perform experiments and record data - Follow established research protocols. Understand the scientific principles involved in the research. Apply those principles to troubleshoot and modify procedures and techniques when appropriate. Maintain accurate and detailed records of all laboratory work and experiments. Analyze data and perform statistical and graphical analysis of data. Design and execute daily laboratory experiments in coordination with senior laboratory personnel. Apply standard protocols to specific experiments. Assists in training post doctoral fellows, graduate students, and junior laboratory personnel in laboratory techniques and equipment. Capable of directing an undergraduate trainee in simple, well-established procedures. Maintains an active role in laboratory's meetings, presentations, and publications. Contributes material for reports, publications, journal articles, and abstracts. Able to orally present data in group presentations.

• Research and improve current methods - Utilize internal and external resources to reproduce, evaluate, research, and improve techniques, bring in new techniques or modify existing techniques. Evaluate innovative techniques

• Serve as a Resource - Demonstrate competence in specific knowledge, skills and behaviors. Effectively communicate with team members and contribute to their growth by sharing knowledge, experience, and skills. Stay abreast of changes in key systems. Serve as a resource to the department in specific area(s).

• Lab Administration -Troubleshoot and correct erroneous results or problems with laboratory equipment. Troubleshoot and/or make modifications as necessary to established procedures. Maintain inventory of laboratory supplies and equipment. Maintain cell cultures. Operate standard laboratory equipment. Willing and able to assist in daily operations of the laboratory including answering phone, maintaining lab supplies, organization, and cleanliness. Maintains optimal protocol and assay accuracy by conducting regular quality checks. Provides appropriate safety monitoring checks as assigned (radiation safety, biosafety, etc.)

• Customer Service - Maintain/manage strong working relationships with clients, colleagues, and other key stakeholders. Respond to requests quickly and effectively. Identify and understand customer needs and expectations and meets them. Communicate any relevant information to clients, colleagues, and other key stakeholders

JOB QUALIFICATIONS

• Required for RAIII:

  ·Bachelor's degree in a related field AND 1 year of directly related experience OR Master's degree in a related field

  Required for RAIV:

  · Bachelor's degree in a related field

  · 3 years of experience in related job discipline OR Master's degree and 2 years experience

• Expected Starting Hourly Rate: 24.30 - 28.46 (RAIII) 26.82 – 33.21 (RAIV)

Primary Location

Location S

Schedule

Full time

Shift

Day (United States of America)

Department

Mol. Cardio. Biology

Employee Status

Regular

FTE

1

Weekly Hours

40

• Medical coverage starting day one of employment. View employee benefits here.

• Competitive retirement plans

• Tuition reimbursement for continuing education

• Expansive employee discount programs through our many community partners

• Shift Differential, Weekend Differential, and Weekend Option Pay Programs for qualified positions

• Support through Employee Resource Groups such as African American Professionals Advisory Council, Asian Cultural and Professional Group, EQUAL - LGBTQA Resource Group, Juntos - Hispanic/Latin Resource Group, Veterans and Military Family Advocacy Network, and Young Professionals (YP) Resource Group

• Physical and mental health wellness programs

• Relocation assistance available for qualified positions

*Benefits may vary based on FTE Status and Position Type

At Cincinnati Children’s, we come to work with one goal: to make children’s health better. We believe in a holistic team approach, both in caring for patients and their families, and in advancing science and discovery. We strive to do better and find energy and inspiration in our shared purpose. If you want to be the best you can be, you can do it at Cincinnati Children’s.

Cincinnati Children's is:

• Recognized by U.S. News & World Report as a top 10 best Children's Hospitals in the nation for more than 15 years

• Consistently among the top 3 Children's Hospitals for National Institutes of Health (NIH) Funding

• Recognized as one of America’s Best Large Employers (2025), America’s Best Employers for New Grads (2025)

• One of the nation's America’s Most Innovative Companies as noted by Fortune

• Consistently certified as great place to work

• A Leading Disability Employer as noted by the National Organization on Disability

• Magnet® designated for the fourth consecutive time by the American Nurses Credentialing Center (ANCC)

We Embrace Innovation—Together. We believe in empowering our teams with the tools that help us work smarter and care better. That’s why we support the responsible use of artificial intelligence. By encouraging innovation, we’re creating space for new ideas, better outcomes, and a stronger future—for all of us.

Comprehensive job description provided upon request.

Cincinnati Children’s is proud to be an Equal Opportunity Employer committed to creating an environment of dignity and respect for all our employees, patients, and families. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, age, genetic information, national origin, sexual orientation, gender identity, disability or protected veteran status. EEO/Veteran/Disability